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Double-Stranded RNA Binding Proteins in Serum Contribute to Systemic RNAi Across Phyla—Towards Finding the Missing Link in Achelata3.17 MB
Published VersionCC BY V4.0, Open Access
Journal article
Double-Stranded RNA Binding Proteins in Serum Contribute to Systemic RNAi Across Phyla—Towards Finding the Missing Link in Achelata
International Journal of Molecular Sciences, Vol.21(18), pp.1-20
2020
Abstract
RNA interference (RNAi) has become a widely utilized method for studying gene function, yet despite this many of the mechanisms surrounding RNAi remain elusive. The core RNAi machinery is relatively well understood, however many of the systemic mechanisms, particularly double-stranded RNA (dsRNA) transport, are not. Here, we demonstrate that dsRNA binding proteins in the serum contribute to systemic RNAi and may be the limiting factor in RNAi capacity for species such as spiny lobsters, where gene silencing is not functional. Incubating sera from a variety of species across phyla with dsRNA led to a gel mobility shift in species in which systemic RNAi has been observed, with this response being absent in species in which systemic RNAi has never been observed. Proteomic analysis suggested lipoproteins may be responsible for this phenomenon and may transport dsRNA to spread the RNAi signal systemically. Following this, we identified the same gel shift in the slipper lobster Thenus australiensis and subsequently silenced the insulin androgenic gland hormone, marking the first time RNAi has been performed in any lobster species. These results pave the way for inducing RNAi in spiny lobsters and for a better understanding of the mechanisms of systemic RNAi in Crustacea, as well as across phyla.
Details
- Title
- Double-Stranded RNA Binding Proteins in Serum Contribute to Systemic RNAi Across Phyla—Towards Finding the Missing Link in Achelata
- Authors
- Thomas M Banks (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - LegacyTianfang Wang (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - LegacyQuinn P Fitzgibbon (Author) - University of TasmaniaGregory G Smith (Author) - University of TasmaniaTomer Ventura (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - Legacy
- Publication details
- International Journal of Molecular Sciences, Vol.21(18), pp.1-20
- Publisher
- MDPI AG
- Date published
- 2020
- DOI
- 10.3390/ijms21186967
- ISSN
- 1422-0067; 1661-6596
- Copyright note
- Copyright (c) 2020 The Authors. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
- Organisation Unit
- School of Science and Engineering - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99482308402621
- Output Type
- Journal article
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- Domestic collaboration
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Chemistry, Multidisciplinary
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