Journal article
Compositional and temporal stability of fecal taxon libraries for use with SourceTracker in sub-tropical catchments
Water Research, Vol.165, 114967
2019
Abstract
Characterization of microbial communities using high-throughput amplicon sequencing is an emerging approach for microbial source tracking of fecal pollution. This study used SourceTracker software to examine temporal and geographical variability of fecal bacterial community profiles to identify pollutant sources in three freshwater catchments in sub-tropical Australia. Fecal bacterial communities from 10 animal species, humans, and composite wastewater samples from six sewage treatment plants were characterized and compared to freshwater samples using Illumina amplicon sequencing of the V5-V6 regions of the 16S rRNA gene. Source contributions were calculated in SourceTracker using new fecal taxon libraries as well as previously generated libraries to determine the effects of geographic and temporal variability on source assignments. SourceTracker determined 16S rRNA bacterial communites within freshwater samples, shared taxonomic similarities to that of wastewater at low levels (typically <3%). SourceTraker also predicted occasional fecal detection of deer and flying fox sources in the water samples. No significant differences in source contributions were observed within sequences from current and previously characterized fecal samples (P ≥ 0.107). However, significant differences were observed between previously characterized and newly characterized source communities (ANOSIM P ≤ 0.001), which shared <15% community composition. Results suggest temporal instability of fecal taxon libraries among tested sources and highlight continual evaluation of community-based MST using confirmatory qPCR analyses of marker genes.
Details
- Title
- Compositional and temporal stability of fecal taxon libraries for use with SourceTracker in sub-tropical catchments
- Authors
- Christian A O'Dea (Author) - University of the Sunshine Coast - School of Health and Sport SciencesQian Zhang (Author) - University of Minnesota, United StatesChristopher Staley (Author) - University of Minnesota, United StatesNicole Masters (Author) - University of the Sunshine Coast - School of Health and Sport SciencesAnna V Kuballa (Author) - University of the Sunshine Coast - School of Health and Sport SciencesPaul Fisher (Author) - SeqwaterCameron Veal (Author) - SeqwaterHelen Stratton (Author) - Griffith UniversityMichael J Sadowsky (Author) - University of MinnesotaWarish Ahmed (Author) - CSIRO Land & WaterMohammad Katouli (Author) - University of the Sunshine Coast - School of Health and Sport Sciences
- Publication details
- Water Research, Vol.165, 114967
- Publisher
- IWA Publishing
- Date published
- 2019
- DOI
- 10.1016/j.watres.2019.114967
- ISSN
- 0043-1354; 0043-1354
- Organisation Unit
- School of Health - Biomedicine; School of Health; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Health and Sport Sciences - Legacy; Forest Industries Research Centre; School of Science, Technology and Engineering; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99451403702621
- Output Type
- Journal article
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