Asymptomatic infections with highly polymorphic Chlamydia suis are ubiquitous in pigs

Min Li; Martina Jelocnik; F Yang; J Gong; B Kaltenboeck; Adam Polkinghorne; Z Feng; Y Pannekoek; N Borel; C Song; P Jiang; Jing Li; J Zhang; Y Wang; J Wang; X Zhou; C Wang

doi:10.1186/s12917-017-1295-x

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Asymptomatic infections with highly polymorphic Chlamydia suis are ubiquitous in pigs

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Asymptomatic infections with highly polymorphic Chlamydia suis are ubiquitous in pigs

Min Li, Martina Jelocnik, F Yang, J Gong, B Kaltenboeck, Adam Polkinghorne, Z Feng, Y Pannekoek, N Borel, C Song, …

BMC Veterinary Research, Vol.13(1), 370

2017

DOI: https://doi.org/10.1186/s12917-017-1295-x

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Abstract

chlamydia suis

FRET-PCR

MLST

ompA

pig

tanglegram

Background: Chlamydia suis is an important, globally distributed, highly prevalent and diverse obligate intracellular pathogen infecting pigs. To investigate the prevalence and genetic diversity of C. suis in China, 2,137 nasal, conjunctival, and rectal swabs as well as whole blood and lung samples of pigs were collected in 19 regions from ten provinces of China in this study. Results: We report an overall positivity of 62.4% (1,334/2,137) of C. suis following screening by Chlamydia spp. 23S rRNA-based FRET-PCR and high-resolution melting curve analysis and confirmatory sequencing. For C. suis-positive samples, 33.3 % of whole blood and 62.5% of rectal swabs were found to be positive for the C. suis tetR(C) gene, while 13.3% of whole blood and 87.0% of rectal swabs were positive for the C. suis tet(C) gene. Phylogenetic comparison of partial C. suis ompA gene sequences revealed significant genetic diversity in the C. suis strains. This genetic diversity was confirmed by C. suis-specific multilocus sequence typing (MLST), which identified 26 novel sequence types among 27 examined strains. Tanglegrams based on MLST and ompA sequences provided evidence of C. suis recombination amongst the strains analyzed. Conclusions: Genetically highly diverse C. suis strains are exceedingly prevalent in pigs. As it stands, the potential pathogenic effect of C. suis on pig health and production of C. suis remains unclear and will be the subject of further investigations. Further study is also required to address the transmission of C. suis between pigs and the risk of 'spill-over' and 'spill-back' of infections to wild animals and humans. © 2017 The Author(s).

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Title: Asymptomatic infections with highly polymorphic Chlamydia suis are ubiquitous in pigs
Authors: Min Li (Author) - Yangzhou University College of Veterinary Medicine, China
Martina Jelocnik (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
F Yang (Author) - Yangzhou University College of Veterinary Medicine, China
J Gong (Author) - Chinese Academy of Agricultural Sciences, China
B Kaltenboeck (Author) - Auburn University, United States
Adam Polkinghorne (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Z Feng (Author) - National Center for Engineering Research of Veterinary Bio-Products, China
Y Pannekoek (Author) - University of Amsterdam, Netherlands
N Borel (Author) - University of Zurich, Switzerland
C Song (Author) - Yunnan Agricultural University College of Animal Science & Technology, China
P Jiang (Author) - Nanjing Agricultural University, China
Jing Li (Author) - Yangzhou University College of Veterinary Medicine, China
J Zhang (Author) - Yangzhou University College of Veterinary Medicine, China
Y Wang (Author) - Yangzhou University College of Veterinary Medicine, China
J Wang (Author) - Yangzhou University College of Veterinary Medicine, China
X Zhou (Author) - Yangzhou University College of Veterinary Medicine, China
C Wang (Author) - Yangzhou University College of Veterinary Medicine, China
Publication details: BMC Veterinary Research, Vol.13(1), 370; 14
Publisher: BioMed Central Ltd.
Date published: 2017
DOI: 10.1186/s12917-017-1295-x
ISSN: 1746-6148; 1746-6148
Copyright note: Copyright © The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Organisation Unit: University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99451073902621
Output Type: Journal article
Research Statement: false

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