Nano-hard template synthesis of pure mesoporous NiO and its application for streptavidin protein immobilization

Mohammad A Wahab; F Darain

doi:10.1088/0957-4484/25/16/165701

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Nano-hard template synthesis of pure mesoporous NiO and its application for streptavidin protein immobilization

Journal article

Peer reviewed

Nano-hard template synthesis of pure mesoporous NiO and its application for streptavidin protein immobilization

Mohammad A Wahab and F Darain

Nanotechnology, Vol.25(16)

2014

DOI: https://doi.org/10.1088/0957-4484/25/16/165701

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Abstract

A simple and efficient immobilization of streptavidin protein (with hexa-histidine tag) onto the surface of mesoporous NiO is described. Before immobilization of streptavidin protein (with hexa-histidine tag) onto the surface of mesoporous NiO, we first synthesized well-organized mesoporous NiO by a nanocasting method using mesoporous silica SBA-15 as the hard template. Then, the well-organized mesoporous NiO particles were characterized by small angle x-ray diffraction (XRD), wide angle XRD, nitrogen adsorption/desorption, and transmission electron microscopy (TEM). TEM and small angle XRD suggested the formation of mesoporous NiO materials, whereas the wide angle XRD pattern of mesoporous NiO indicated that the nickel precursor had been transformed into crystalline NiO. The N2 sorption experiments demonstrated that the mesoporous NiO particles had a high surface area of 281 m2 g-1, a pore volume of 0.51 cm3 g-1 and a pore size of 4.8 nm. Next, the immobilization of streptavidin protein (with hexa-histidine tag) onto the surface of mesoporous NiO was studied. Detailed analysis using gel electrophoresis confirmed that this approach can efficiently bind his-tagged streptavidin onto the surface of mesoporous NiO material since the mesoporous NiO provides sufficient surface sites for the binding of streptavidin via non-covalent ligand binding with the histidine tag. © 2014 IOP Publishing Ltd.

Details

Title: Nano-hard template synthesis of pure mesoporous NiO and its application for streptavidin protein immobilization
Authors: Mohammad A Wahab (Author) - University of Queensland
F Darain (Author) - University of Queensland
Publication details: Nanotechnology, Vol.25(16)
Publisher: Institute of Physics Publishing Ltd.
Date published: 2014
DOI: 10.1088/0957-4484/25/16/165701
ISSN: 0957-4484
Organisation Unit: University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99451063302621
Output Type: Journal article

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Web Of Science research areas: Materials Science, Multidisciplinary; Nanoscience & Nanotechnology; Physics, Applied