Channel gating in the absence of agonist by a homooligomeric molluscan GABA receptor expressed in Xenopus oocytes from a cloned cDNA

N S Bhandal; R L Ramsey; Robert J Harvey; M G Darlison; P N R Usherwood

doi:10.1007/BF02211028

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Channel gating in the absence of agonist by a homooligomeric molluscan GABA receptor expressed in Xenopus oocytes from a cloned cDNA

Journal article

Peer reviewed

Channel gating in the absence of agonist by a homooligomeric molluscan GABA receptor expressed in Xenopus oocytes from a cloned cDNA

N S Bhandal, R L Ramsey, Robert J Harvey, M G Darlison and P N R Usherwood

Invertebrate Neuroscience, Vol.1(3), pp.267-272

1995

DOI: https://doi.org/10.1007/BF02211028

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Abstract

GABA receptors

mollusc (Lymnaea stagnalis)

picrotoxin

single-channel recordings

spontaneous channel openings

Xenopus oocytes

We have previously described the isolation of a complementary DNA (cDNA) from the freshwater mollusc Lymnaea stagnalis encoding a polypeptide that exhibits ∼50% identity to the ÃŸ-subunits of vertebrate γ-aminobutyric acid (GABA) type A (GABAA) receptor. When expressed in Xenopus laevis oocytes from in vitro-transcribed RNA, the snail subunit forms functional homo-oligomeric receptors possessing chloride-selective ion channels. In recordings from voltage-clamped oocytes held at -60 mV, GABA induced an inward current, whereas application of the chloride-channel blocker picrotoxin (in the absence of agonist) elicited an apparent outward current. Single channel recordings obtained from cell-attached patches have revealed a single population of ∼20 pS channels, with an open probability greater than 90% (at a pipette potential of -100 mV) in the absence of GABA. The relationship between single channel current and pipette potential was linear over the studied range (-100 mV to +60 mV), but the open probability was less for hyperpolarizations than for depolarizations. The spontaneous channel openings were blocked by micromolar concentrations of picrotoxin. Functional hetero-oligomeric receptors were formed when the molluscan subunit was co-expressed in oocytes with the bovine GABAA receptor α1-subunit, but the channels gated by these receptors did not open spontaneously. © 1995 Sheffield Academic Press.

Details

Title: Channel gating in the absence of agonist by a homooligomeric molluscan GABA receptor expressed in Xenopus oocytes from a cloned cDNA
Authors: N S Bhandal (Author) - University of Nottingham, United Kingdom
R L Ramsey (Author) - University of Nottingham, United Kingdom
Robert J Harvey (Author) - Universität Hamburg, Germany
M G Darlison (Author) - Universität Hamburg, Germany
P N R Usherwood (Author) - University of Nottingham, United Kingdom
Publication details: Invertebrate Neuroscience, Vol.1(3), pp.267-272
Publisher: Springer
Date published: 1995
DOI: 10.1007/BF02211028
ISSN: 1354-2516
Organisation Unit: School of Health; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99450543502621
Output Type: Journal article

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