An epidermal-specific ethylene signal cascade regulates trans-differentiation of transfer cells in Vicia faba cotyledons

Yuchan Zhou; F Andriunas; C E Offler; D W McCurdy; J W Patrick

doi:10.1111/j.1469-8137.2009.03136.x

Back

An epidermal-specific ethylene signal cascade regulates trans-differentiation of transfer cells in Vicia faba cotyledons

Journal article

Peer reviewed

An epidermal-specific ethylene signal cascade regulates trans-differentiation of transfer cells in Vicia faba cotyledons

Yuchan Zhou, F Andriunas, C E Offler, D W McCurdy and J W Patrick

New Phytologist, Vol.185(4), pp.931-943

2010

DOI: https://doi.org/10.1111/j.1469-8137.2009.03136.x

Files and links (1)

url

https://doi.org/10.1111/j.1469-8137.2009.03136.xView

Published Version

Abstract

ethylene

signal transduction

transfer cells (TCs)

Vicia faba (faba bean)

wall ingrowths

Transfer cells (TCs) trans-differentiate by developing extensive wall ingrowths that facilitate enhanced plasma membrane transport of nutrients. Signal(s) and signalling cascades responsible for initiating this trans-differentiation event are poorly understood. We tested the hypothesis that ethylene functions as a key inductive signal for wall ingrowth formation in epidermal cells of Vicia faba cotyledons. Scanning electron microscopy of epidermal cells monitored their propensity for wall ingrowth formation. Spatial and temporal expression profiles of ethylene biosynthetic enzymes and key elements of ethylene signalling cascades (ethylene insensitive 3 (EIN3) and ethylene response factors (ERFs)) were determined. Wall-ingrowth formation responded positively to manipulation of ethylene biosynthesis and perception. It was preceded by a cell-specific burst in ethylene biosynthesis accompanied by a co-localized post-translational up-regulation of VfEIN3-1 and differential expression of three VfERF genes. Blocking ethylene production arrested ongoing wall ingrowth development. Wound-induced ethylene in pod walls and seed coats caused an in planta activation of ethylene biosynthetic genes in adaxial epidermal cells that coincidentally formed wall ingrowths. A cell-specific burst of ethylene biosynthesis functions as an inductive signal initiating and sustaining trans-differentiation to a TC morphology in vitro. These events are reproduced for developing V. faba seeds in planta. © The Authors (2010). Journal compilation © New Phytologist (2010).

Details

Title: An epidermal-specific ethylene signal cascade regulates trans-differentiation of transfer cells in Vicia faba cotyledons
Authors: Yuchan Zhou (Author) - University of Newcastle
F Andriunas (Author) - University of Newcastle
C E Offler (Author) - University of Newcastle
D W McCurdy (Author) - University of Newcastle
J W Patrick (Author) - University of Newcastle
Publication details: New Phytologist, Vol.185(4), pp.931-943
Publisher: Wiley-Blackwell Publishing Ltd.
Date published: 2010
DOI: 10.1111/j.1469-8137.2009.03136.x
ISSN: 0028-646X
Organisation Unit: Australian Centre for Pacific Islands Research; University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99449918602621
Output Type: Journal article

Metrics

2 File views/ downloads

439 Record Views

36 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Web Of Science research areas: Plant Sciences

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites