Wide range of Chlamydiales types detected in native Australian mammals

T J Bodetti; K Viggers; K Warren; R Swan; S Conaghty; C Sims; Peter Timms

doi:10.1016/S0378-1135(03)00211-6

Back

Wide range of Chlamydiales types detected in native Australian mammals

Journal article

Peer reviewed

Wide range of Chlamydiales types detected in native Australian mammals

T J Bodetti, K Viggers, K Warren, R Swan, S Conaghty, C Sims and Peter Timms

Veterinary Microbiology, Vol.96(2), pp.177-187

2003

DOI: https://doi.org/10.1016/S0378-1135(03)00211-6

Files and links (1)

url

https://doi.org/10.1016/S0378-1135(03)00211-6View

Published Version

Abstract

chlamydiales

marsupiales

Australia

The Chlamydiales are a unique order of intracellular bacterial pathogens that cause significant disease of birds and animals, including humans. The recent development of a Chlamydiales-specific 16S rDNA polymerase chain reaction (PCR) assay has enabled the identification of Chlamydiales DNA from an increasing range of hosts and environmental sources. Whereas the Australian marsupial, the koala, has previously been shown to harbour several Chlamydiales types, no other Australian marsupials have been analysed. We therefore used a 16S rDNA PCR assay combined with direct sequencing to determine the presence and genotype of Chlamydiales in five wild Australian mammals (gliders, possums, bilbies, bandicoots, potoroos). We detected eight previously observed Chlamydiales genotypes as well as 10 new Chlamydiales sequences from these five Australian mammals. In addition to PCR analysis we used antigen specific staining and in vitro culture in HEp-2 cell monolayers to confirm some of the identifications. A strong association between ocular PCR positivity and the presence of clinical disease (conjunctivitis, proliferation of the eyelid) was observed in two of the species studied, gliders and bandicoots, whereas little clinical disease was observed in the other animals studied. These findings provide further evidence that novel Chlamydiales infections occur in a wide range of hosts and that, in some of these, the chlamydial infections may contribute to clinical disease. © 2003 Elsevier B.V. All rights reserved.

Details

Title: Wide range of Chlamydiales types detected in native Australian mammals
Authors: T J Bodetti (Author) - Queensland University of Technology
K Viggers (Author) - Australian National University
K Warren (Author) - Murdoch University
R Swan (Author) - Murdoch University
S Conaghty (Author) - Monarto Zoological Park
C Sims (Author) - Department of Conservation and Land Management
Peter Timms (Author) - Queensland University of Technology
Publication details: Veterinary Microbiology, Vol.96(2), pp.177-187
Publisher: Elsevier BV
Date published: 2003
DOI: 10.1016/S0378-1135(03)00211-6
ISSN: 0378-1135
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99449806402621
Output Type: Journal article

Metrics

549 Record Views

41 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration
Web Of Science research areas: Microbiology; Veterinary Sciences

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites