Journal article
Gene Expression Profiling of the Cephalothorax and Eyestalk in Penaeus Monodon during Ovarian Maturation
International Journal of Biological Sciences, Vol.8(3), pp.328-343
2012
Abstract
In crustaceans, a range of physiological processes involved in ovarian maturation occurs in organs of the cephalothorax including the hepatopancrease, mandibular and Y-organ. Addi-tionally, reproduction is regulated by neuropeptide hormones and other proteins released from secretory sites within the eyestalk. Reproductive dysfunction in captive-reared prawns, Penaeus monodon, is believed to be due to deficiencies in these factors. In this study, we in-vestigated the expression of gene transcripts in the cephalothorax and eyestalk from wild-caught and captive-reared animals throughout ovarian maturation using custom oligo-nucleotide microarray screening. We have isolated numerous transcripts that appear to be differentially expressed throughout ovarian maturation and between wild-caught and cap-tive-reared animals. In the cephalothorax, differentially expressed genes included the 1,3-β-D-glucan-binding high-density lipoprotein, 2/3-oxoacyl-CoA thiolase and vitellogenin. In the eyestalk, these include gene transcripts that encode a protein that modulates G-protein coupled receptor activity and another that encodes an architectural transcription factor. Each may regulate the expression of reproductive neuropeptides, such as the crustacean hyper-glycaemic hormone and molt-inhibiting hormone. We could not identify differentially ex-pressed transcripts encoding known reproductive neuropeptides in the eyestalk of either wild-caught or captive-reared prawns at any ovarian maturation stage, however, this result may be attributed to low relative expression levels of these transcripts. In summary, this study provides a foundation for the study of target genes involved in regulating penaeid reproduc-tion.
Details
- Title
- Gene Expression Profiling of the Cephalothorax and Eyestalk in Penaeus Monodon during Ovarian Maturation
- Authors
- P Brady (Author) - University of QueenslandAbigail Elizur (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringR Williams (Author) - University of QueenslandScott F Cummins (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringWayne R Knibb (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- International Journal of Biological Sciences, Vol.8(3), pp.328-343
- Publisher
- Ivyspring International Publisher
- Date published
- 2012
- DOI
- 10.7150/ijbs.3517
- ISSN
- 1449-2288
- Copyright note
- Copyright © 2012 Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/ licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99449797602621
- Output Type
- Journal article
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