Journal article
Efficiency of transcription from promoter sequence variants in Lactobacillus is both strain and context dependent
Journal of Bacteriology, Vol.181(20), pp.6569-6572
1999
Abstract
The introduction of consensus -35 (TTGACA) and -10 (TATAAT) hexamers and a TG motif into the Lactobacillus acidophilus ATCC 4356 wild-type slpA promoter resulted in significant improvements (4.3-, 4.1-, and 10.7-fold, respectively) in transcriptional activity in Lactobacillus fermentum BR11. In contrast, the same changes resulted in decreased transcription in Lactobacillus rhamnosus GG. The TG motif was shown to be important in the context of weak -35 and -10 hexamers (L. fermentum BR11) or a consensus - 10 hexamer (L. rhamnosus GG). Thus, both strain- and context-dependent effects are critical factors influencing transcription in Lactobacillus.
Details
- Title
- Efficiency of transcription from promoter sequence variants in Lactobacillus is both strain and context dependent
- Authors
- A McCracken (Author) - Queensland University of TechnologyPeter Timms (Author) - Queensland University of Technology
- Publication details
- Journal of Bacteriology, Vol.181(20), pp.6569-6572
- Publisher
- American Society for Microbiology
- Date published
- 1999
- DOI
- 10.1128/JB.181.20.6569-6572.1999
- ISSN
- 0021-9193; 0021-9193
- Copyright note
- Copyright © 1999 American Society for Microbiology. Reproduced here in accordance with the publishers copyright policy.
- Organisation Unit
- University of the Sunshine Coast, Queensland; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99449620102621
- Output Type
- Journal article
- Research Statement
- false
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