Journal article
Visualising DNA in classrooms using Nile Blue
Teaching Science, Vol.54(4), pp.31-33
2008
Abstract
Giving students the opportunity to extract, manipulate and visualise DNA molecules enhances a constructivist approach to learning about modern techniques in biology and biotechnology Visualisation usually requires agarose gel electrophoresls and staining. In this article, we report on an alternative DNA stain, Nile Blue A, that may be used In the classroom to visualise DNA. The stain is inexpensive, is at least as sensitive as methylene blue, does not require destaining, requires visualisation with only visible light, and is specific for DNA but not RNA.
Details
- Title
- Visualising DNA in classrooms using Nile Blue
- Authors
- C Milne (Author) - Queensland Department of Education, Training and the ArtsScott Roche (Author) - University of the Sunshine Coast - Faculty of Science, Health and EducationDavid B McKay (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
- Publication details
- Teaching Science, Vol.54(4), pp.31-33
- Publisher
- Australian Science Teachers Association
- Date published
- 2008
- ISSN
- 1449-6313
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
- Language
- English
- Record Identifier
- 99449511702621
- Output Type
- Journal article
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