Journal article
Investigation of antibacterial phytochemicals in the bark and leaves of Ficus coronata by high-performance liquid chromatography-electrospray ionization–ion trap mass spectrometry (HPLC-ESI-MSn) and ESI-MSn
Electrophoresis, Vol.33(4), pp.713-718
2012
Abstract
The ethyl acetate extracts of the bark and leaves of Ficus coronata were separated by column chromatography and the resulting fractions tested for their bioactivity toward methicillin-resistant-Staphylococcus aureus (MRSA) and M. luteus. The bioactive column chromatography fractions were further separated by preparative thin layer chromatography (TLC) and the resulting bands investigated by high-performance liquid chromatography-electrospray ionization-ion trap mass spectrometry (HPLC-ESI-MSn) and ESI-MSn. The resulting retention times, molecular masses, their fragmentation patterns, and the chemnet database (www.chemnetbase.com) were then used in the dereplication process by structural elucidation of some of the compounds when compared with known structures of natural origin. Some molecular masses and the corresponding fragmentations were found that did not correlate with any known compounds thus revealing potentially novel natural products that could be investigated on a larger scale and could ultimately find application as new drugs against MRSA and other multidrug-resistant microorganisms. Structures are also proposed for known compounds that have not been previously reported for F. coronata.
Details
- Title
- Investigation of antibacterial phytochemicals in the bark and leaves of Ficus coronata by high-performance liquid chromatography-electrospray ionization–ion trap mass spectrometry (HPLC-ESI-MSn) and ESI-MSn
- Authors
- T J Smyth (Author) - University of Ulster, United KingdomV N Ramachandran (Author) - University of Ulster, United KingdomPeter R Brooks (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringW F Smyth (Author) - University of Ulster, United Kingdom
- Publication details
- Electrophoresis, Vol.33(4), pp.713-718
- Publisher
- Wiley - V C H Verlag GmbH & Co. KGaA
- Date published
- 2012
- DOI
- 10.1002/elps.201100302
- ISSN
- 0173-0835; 0173-0835
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99449440702621
- Output Type
- Journal article
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