Serological diagnosis of Leptospirosis in bovine serum samples using a microsphere immunoassay

Sarah Jane Wynwood; M A Burns; Glenn C Graham; S L Weier; David B McKay; Scott B Craig

doi:10.1136/vetreco-2015-000148

Back

Serological diagnosis of Leptospirosis in bovine serum samples using a microsphere immunoassay

Journal article

Open access

Peer reviewed

Serological diagnosis of Leptospirosis in bovine serum samples using a microsphere immunoassay

Sarah Jane Wynwood, M A Burns, Glenn C Graham, S L Weier, David B McKay and Scott B Craig

Veterinary Record Open, Vol.3(1), e000148

2016

DOI: https://doi.org/10.1136/vetreco-2015-000148

Files and links (2)

pdf

PDF - Published Version (Open Access)914.31 kBDownload View

Published VersionPDF - Published Version (Open Access)CC BY-NC V4.0, Open Access

url

https://doi.org/10.1136/vetreco-2015-000148View

Published Version

Abstract

Leptospirosis causes significant economic loss within the cattle industry worldwide. Current diagnostic methods are generally inadequate for dealing with large numbers of samples, are outdated, and provide little useful diagnostic and epidemiological information. This aim of this study was to apply a microsphere immunoassay (MIA), utilising Luminex xMap technology, to 200 bovine serum samples to determine this method's usefulness in leptospirosis diagnosis in comparison with the current gold standard, the microscopic agglutination test (MAT). Although MAT is the most widely used laboratory test for the diagnosis of leptospirosis, its reliance on live cultures, subjective interpretation of results and an inability to differentiate between antibody classes, suggest MAT is no longer the best method for the diagnosis of leptospirosis. The results presented in this paper show that MIA was able to determine reactive from non-reactive samples when compared with MAT, and was able to differentiate IgG and IgM classes of antibody. The results suggest increased sensitivity in MIA and the ability to multiplex up to 500 antigens at one time allows for significant improvements in cost-effectiveness as well as a reduced dependency on live cultures. The relatively low cost, high throughput platform and differentiation of antibody class, as shown in previous research, make this assay worthy of consideration for the diagnosis of leptospirosis in small-scale or large-scale bovine populations.

Details

Title: Serological diagnosis of Leptospirosis in bovine serum samples using a microsphere immunoassay
Authors: Sarah Jane Wynwood (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
M A Burns (Author) - Queensland Health Forensic and Scientific Services
Glenn C Graham (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
S L Weier (Author) - Queensland University of Technology
David B McKay (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Scott B Craig (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: Veterinary Record Open, Vol.3(1), e000148; 7
Publisher: British Veterinary Association
Date published: 2016
DOI: 10.1136/vetreco-2015-000148
ISSN: 2052-6113
Copyright note: Copyright © 2016 The Authors. This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work noncommercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http:// creativecommons.org/licenses/by-nc/4.0/
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
Language: English
Record Identifier: 99449357502621
Output Type: Journal article

Metrics

94 File views/ downloads

1261 Record Views