Journal article
The impact of delayed blood centrifuging, choice of collection tube, and type of assay on 25-hydroxyvitamin D concentrations
Cancer Causes and Control, Vol.21(4), pp.643-648
2010
Abstract
Studies have examined the associations between cancers and circulating 25-hydroxyvitamin D [25(OH)D], but little is known about the impact of different laboratory practices on 25(OH)D concentrations. We examined the potential impact of delayed blood centrifuging, choice of collection tube, and type of assay on 25(OH)D concentrations. Blood samples from 20 healthy volunteers underwent alternative laboratory procedures: four centrifuging times (2, 24, 72, and 96 h after blood draw); three types of collection tubes (red top serum tube, two different plasma anticoagulant tubes containing heparin or EDTA); and two types of assays (DiaSorin radioimmunoassay [RIA] and chemiluminescence immunoassay [CLIA/LIAISON® Log-transformed 25(OH)D concentrations were analyzed using the generalized estimating equations (GEE) linear regression models. We found no difference in 25(OH)D concentrations by centrifuging times or type of assay. There was some indication of a difference in 25(OH)D concentrations by tube type in CLIA/LIAISON®-assayed samples, with concentrations in heparinized plasma (geometric mean, 16.1 ng ml-1) higher than those in serum (geometric mean, 15.3 ng ml-1) (p = 0.01), but the difference was significant only after substantial centrifuging delays (96 h). Our study suggests no necessity for requiring immediate processing of blood samples after collection or for the choice of a tube type or assay. © 2009 US Government.
Details
- Title
- The impact of delayed blood centrifuging, choice of collection tube, and type of assay on 25-hydroxyvitamin D concentrations
- Authors
- C L Yu (Author) - National Institute of Health, United StatesR T Falk (Author) - National Institute of Health, United StatesMichael G Kimlin (Author) - Queensland University of TechnologyP Rajaraman (Author) - National Institute of Health, United StatesA J Sigurdson (Author) - National Institute of Health, United StatesR L Horst (Author) - Heartland Assays, Inc, United StatesL M Cosentino (Author) - SAIC-Frederick Inc., United StatesM S Linet (Author) - National Institute of Health, United StatesD M Freedman (Author) - National Institute of Health, United States
- Publication details
- Cancer Causes and Control, Vol.21(4), pp.643-648
- Publisher
- Springer Netherlands
- Date published
- 2010
- DOI
- 10.1007/s10552-009-9485-x
- ISSN
- 0957-5243; 0957-5243
- Copyright note
- Copyright © 2010 Springer Netherlands. The author's accepted version is reproduced here in accordance with the publisher's copyright policy. The final publication is available at Springer via http://dx.doi.org/10.1007/s10552-009-9485-x
- Organisation Unit
- University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy
- Language
- English
- Record Identifier
- 99449254502621
- Output Type
- Journal article
- Research Statement
- false
Metrics
186 File views/ downloads
653 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Industry collaboration
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Oncology
- Public, Environmental & Occupational Health
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites