TCam-2 seminoma cell line exhibits characteristic foetal germ cell responses to TGF-beta ligands and retinoic acid

J C Young; A Jaiprakash; S Mithraprabhu; Catherine Itman; R Kitazawa; L H J Looijenga; K L Loveland

doi:10.1111/j.1365-2605.2011.01170.x

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TCam-2 seminoma cell line exhibits characteristic foetal germ cell responses to TGF-beta ligands and retinoic acid

Journal article

Peer reviewed

TCam-2 seminoma cell line exhibits characteristic foetal germ cell responses to TGF-beta ligands and retinoic acid

J C Young, A Jaiprakash, S Mithraprabhu, Catherine Itman, R Kitazawa, L H J Looijenga and K L Loveland

International Journal of Andrology, Vol.34(4, Part 2), pp.e204-e217

2011

DOI: https://doi.org/10.1111/j.1365-2605.2011.01170.x

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Abstract

activin

activin receptors

BMP

testis cancer

Germ cell testicular cancer is understood to arise during embryogenesis, based on the persistence of embryonic germ cell markers in carcinoma in situ and seminoma. In this study, we examine the potential of the seminoma-derived TCam-2 cell line to be used as representative in functional analyses of seminoma. We demonstrate expression of several early germ cell markers, including BLIMP1, OCT3/4, AP2γ, NANOG and KIT. Many TGF-beta superfamily receptors and downstream transcription factors are also present in these cells including the normally foetal ACTRIIA receptor, indicating potential responsiveness to TGF-beta superfamily ligands. Treatment with BMP4 or RA induces a significant increase in ACTRIA, ACTRIIA and ACTRIIB transcripts, whereas activin A decreases ACTRIB. BMP4 and RA each support TCam-2 survival and/or proliferation. In addition, despite increased KIT mRNA levels induced by BMP4, RA and activin A, activin A does not improve survival or proliferation. The capacity for BMP4 and retinoic acid to enhance foetal germ cell survival and proliferation/self-renewal has been demonstrated in mice, but not previously tested in humans. This study is the first to demonstrate a functional response in seminoma cells, using a well-characterized cell line, consistent with their foetal germ cell-like identity. © 2011 The Authors. International Journal of Andrology © 2011 European Academy of Andrology.

Details

Title: TCam-2 seminoma cell line exhibits characteristic foetal germ cell responses to TGF-beta ligands and retinoic acid
Authors: J C Young (Author) - Monash University
A Jaiprakash (Author) - Monash University
S Mithraprabhu (Author) - Monash University
Catherine Itman (Author) - Monash University
R Kitazawa (Author) - Ehime University Graduate School of Medicine, Japan
L H J Looijenga (Author) - University Medical Centre Rotterdam, Netherlands
K L Loveland (Author) - Monash University
Publication details: International Journal of Andrology, Vol.34(4, Part 2), pp.e204-e217
Publisher: Wiley-Blackwell Publishing Ltd.
Date published: 2011
DOI: 10.1111/j.1365-2605.2011.01170.x
ISSN: 0105-6263; 0105-6263
Organisation Unit: University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99449172202621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Andrology

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