Journal article
Evaluation of the relationship between Chlamydia pecorum sequence types and disease using a species-specific multi-locus sequence typing scheme (MLST)
Veterinary Microbiology, Vol.174(1-2), pp.214-222
2014
Abstract
Chlamydia pecorum is globally associated with several ovine diseases including keratoconjunctivitis and polyarthritis. The exact relationship between the variety of C. pecorum strains reported and the diseases described in sheep remains unclear, challenging efforts to accurately diagnose and manage infected flocks. In the present study, we applied C. pecorum Multi Locus Sequence Typing (MLST) to C. pecorum positive samples collected from sympatric flocks of Australian sheep presenting with conjunctivitis, conjunctivitis with polyarthritis, or polyarthritis only and with no clinical disease (NCD) in order to elucidate the exact relationships between the infecting strains and the range of diseases. Using Bayesian phylogenetic and cluster analyses on 62 C. pecorum positive ocular, vaginal and rectal swab samples from sheep presenting with a range of diseases and in a comparison to C. pecorum sequence types (STs) from other hosts, one ST (ST 23) was recognised as a globally distributed strain associated with ovine and bovine diseases such as polyarthritis and encephalomyelitis. A second ST (ST 69) presently only described in Australian animals, was detected in association with ovine as well as koala chlamydial infections. The majority of vaginal and rectal C. pecorum STs from animals with NCD and/or anatomical sites with no clinical signs of disease in diseased animals, clustered together in a separate group, by both analyses. Furthermore, eight/13 detected STs were novel. This study provides a platform for strain selection for further research into the pathogenic potential of C. pecorum in animals and highlights targets for potential strain-specific diagnostic test development.
Details
- Title
- Evaluation of the relationship between Chlamydia pecorum sequence types and disease using a species-specific multi-locus sequence typing scheme (MLST)
- Authors
- Martina Jelocnik (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringEvelyn Walker (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringYvonne Pannekoek (Author) - Center for Infection and Immunity, Academic Medical Center, NetherlandsJudy Ellem (Author) - North West Local Land ServicesPeter Timms (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringAdam Polkinghorne (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- Veterinary Microbiology, Vol.174(1-2), pp.214-222
- Publisher
- Elsevier BV
- Date published
- 2014
- DOI
- 10.1016/j.vetmic.2014.08.018
- ISSN
- 0378-1135
- Copyright note
- Copyright © 2014. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99449002102621
- Output Type
- Journal article
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- Microbiology
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