Journal article
Variation in mitochondrial minichromosome composition between blood-sucking lice of the genus Haematopinus that infest horses and pigs
Parasites & vectors, Vol.7, 144
2014
Abstract
Background: The genus Haematopinus contains 21 species of blood-sucking lice, parasitizing both eventoed ungulates (pigs, cattle, buffalo, antelopes, camels and deer) and odd-toed ungulates (horses, donkeys and zebras). The mitochondrial genomes of the domestic pig louse, Haematopinus suis, and the wild pig louse, Haematopinus apri, have been sequenced recently; both lice have fragmented mitochondrial genomes with 37 genes on nine minichromosomes. To understand whether the composition of mitochondrial minichromosomes and the gene content and gene arrangement of each minichromosome are stable within the genus, we sequenced the mitochondrial genome of the horse louse, Haematopinus asini. Methods: We used a PCR-based strategy to amplify four mitochondrial minichromosomes in near fulllength, and then amplify the entire coding regions of all of the nine mitochondrial minichromosomes of the horse louse. These amplicons were sequenced with an Illumina Hiseq platform. Results: We identified all of the 37 mitochondrial genes typical of bilateral animals in the horse louse, Haematopinus asini; these genes are on nine circular minichromosomes. Each minichromosome is 3.5-5.0 kb in size and consists of a coding region and a non-coding region except R-nad4L-rrnS-C minichromosome, which contains two coding regions and two non-coding regions. Six of the nine minichromosomes of the horse louse have their counterparts in the pig lice with the same gene content and gene arrangement. However, the gene content and arrangement of the other three minichromosomes of the horse louse, including R-nad4L-rrnS-C, are different from that of the other three minichromosomes of the pig lice. Conclusions: Comparison between the horse louse and the pig lice revealed variation in the composition of mitochondrial minichromosomes within the genus Haematopinus, which can be accounted for by gene translocation events between minichromosomes. The current study indicates that inter-minichromosome recombination plays a major role in generating the variation in the composition of mitochondrial minichromosomes and provides novel insights into the evolution of fragmented mitochondrial genomes in the blood-sucking lice.
Details
- Title
- Variation in mitochondrial minichromosome composition between blood-sucking lice of the genus Haematopinus that infest horses and pigs
- Authors
- Simon Song (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringS C Barker (Author) - University of QueenslandRenfu Shao (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- Parasites & vectors, Vol.7, 144
- Publisher
- BioMed Central Ltd.
- Date published
- 2014
- DOI
- 10.1186/1756-3305-7-144
- ISSN
- 1756-3305
- Copyright note
- Copyright © 2014 Song et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99448835402621
- Output Type
- Journal article
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