http://research.usc.edu.au/vital/access/manager/Index ${session.getAttribute("locale")} 5 Molecular characterisation and expression analysis of Interferon gamma in response to natural Chlamydia infection in the koala, Phascolarctos cinereus http://research.usc.edu.au/vital/access/manager/Repository/usc:12166 Tue 16 Oct 2018 11:23:48 AEST ]]> Influence of putative forest refugia and biogeographic barriers on the level and distribution of genetic variation in an African savannah tree, Khaya senegalensis (Desr.) A. Juss http://research.usc.edu.au/vital/access/manager/Repository/usc:17350 5000 km) and from the steppes of the Sahara desert to the fringes of Africa’s equatorial rainforests (700 km). Three chloroplast (cpDNA) loci and 13 nuclear microsatellite (nSSR) loci were genotyped in 503 individuals. Individual-based clustering of nSSR genotypes identified that all samples formed two populations that showed no pattern of geographic structuring. Population level analysis of nSSR data revealed only very weak genetic structure (F ST = 0.013) with most of the genetic diversity contained within populations. Geographical differentiation (G ST = 0.096) was low for the 14 haplotypes identified. However, whereas all haplotypes occurred in the western populations (including Cameroon), only two cosmopolitan haplotypes were found in central-eastern populations. Geographically restricted low-frequency haplotypes were found in eight western populations. Higher genetic diversity in western populations was confirmed by patterns of allelic richness, which were lower in central-eastern populations. Taken together, these results indicate that K. senegalensis displays very little genetic differentiation across its geographic range and that previously identified barriers to dispersal across the northern savannah belt of tropical Africa (e.g. the Dahomey Gap, Mega Lake Chad and the Adamawa Highlands) have not impeded contemporary gene dispersal in this species. Nevertheless, clustering of rare haplotypes indicates that the Dahomey Gap has likely been an important historical barrier to genetic connectivity between western and eastern regions of the species and that it is likely that this savannah tree also experienced range contraction around major forest refugial fringes or riparian incursions during the last glacial maximum (LGM).]]> Mon 02 Jul 2018 11:56:56 AEST ]]> Allelic variation at a single gene increases food value in a drought-tolerant staple cereal http://research.usc.edu.au/vital/access/manager/Repository/usc:11757 Mon 02 Jul 2018 10:58:14 AEST ]]> Lack of low frequency variants masks patterns of non-neutral evolution following domestication http://research.usc.edu.au/vital/access/manager/Repository/usc:11763 Mon 02 Jul 2018 10:57:17 AEST ]]> Molecular Markers in Plant Improvement http://research.usc.edu.au/vital/access/manager/Repository/usc:11766 Mon 02 Jul 2018 10:57:05 AEST ]]> Whole-genome sequencing reveals untapped genetic potential in Africa’s indigenous cereal crop sorghum http://research.usc.edu.au/vital/access/manager/Repository/usc:11767 Mon 02 Jul 2018 10:57:02 AEST ]]> Characterization and multiplexing of EST-SSR primers in Cynodon (Poaceae) species http://research.usc.edu.au/vital/access/manager/Repository/usc:11746 Mon 02 Jul 2018 10:55:48 AEST ]]> Development and characterization of microsatellite loci for Khaya senegalensis (Meliaceae) http://research.usc.edu.au/vital/access/manager/Repository/usc:11744 Mon 02 Jul 2018 10:55:33 AEST ]]> Isolation and characterisation of novel microsatellite and mitochondrial DNA markers for the Eastern Water Dragon (Physignathus lesueurii) http://research.usc.edu.au/vital/access/manager/Repository/usc:11751 Mon 02 Jul 2018 10:55:00 AEST ]]>