http://research.usc.edu.au/vital/access/manager/Index ${session.getAttribute("locale")} 5 Transcriptomic profiling of host skin immune responses to infestation with Sarcoptes scabieiin a porcine model http://research.usc.edu.au/vital/access/manager/Repository/usc:17396 Wed 26 Oct 2016 11:00:45 AEST ]]> Transcriptional analysis of differential immune and inflammatory responses to Sarcoptes scabiei infestation in a porcine model http://research.usc.edu.au/vital/access/manager/Repository/usc:29154 Wed 21 Aug 2019 14:34:00 AEST ]]> Transcriptional analysis of differential immune responses to Sarcoptes scabiei infestation in a porcine model. http://research.usc.edu.au/vital/access/manager/Repository/usc:26302 1000) of significantly differentially expressed genes were identified at each time point. The analysis revealed numerous genes with roles in allergy and inflammation, including pro-inflammatory cytokines and chemokines involved in immune cell activation and recruitment. In addition, the analysis demonstrated expression of transcripts of various cell surface markers, acute phase proteins, transcription factors, and signaling molecules. Key signaling pathways involved with scabies mite infestation included, “Th1 and Th2 Activation Pathway”, “JAK-STAT Signaling Pathway”, “NF-κB Signaling Pathway”, “Immune Cell Trafficking”, “Communication between Innate and Adaptive Immune Cells”, “Acute Phase Response Signaling” and “Role of IL-17F in Allergic Inflammatory Airway Disease”. In crusted scabies relative to ordinary scabies, we detected gene expression associated with pathophysiologic pathways implicated in psoriasis, atopic dermatitis, rheumatoid arthritis and Th17 (IL17A, ARG1) pronounced responses. To validate the differential gene expression, qRT- PCR confirmation was carried out for selected genes. ELISA analysis was also performed to characterise acute phase response proteins (Haptoglobin, Transferrin, Serum amyloid A and α1 Acid glycoprotein) in serum collected from pigs at selected time points. The analysis demonstrated an increased trend in SAA and significantly higher AGP levels post mite infestation in crusted scabies. In summary, transcriptional profiling revealed genes in the T helper lymphocyte (Th1), Th2 and Th17 signaling pathways, acute phase response signaling, and allergic inflammatory response are associated with scabies. For the first time, we have described key transcriptional changes associated with the development of ordinary scabies and significantly, the distinction between ordinary and crusted scabies. This work provides the basis for follow-up studies in clinical patients with crusted scabies and may provide new control strategies for this severely debilitating disease.]]> Tue 04 Dec 2018 11:22:20 AEST ]]> Host immune responses to the itch mite, Sarcoptes scabiei, in humans http://research.usc.edu.au/vital/access/manager/Repository/usc:23337 Fri 28 Jun 2019 13:24:35 AEST ]]>