${session.getAttribute("locale")} 5 The mRNA levels of GtH Iβ, GtH IIβ and GH in relation to testicular development and testosterone treatment in pituitary cells of male tilapia Tue 16 Oct 2018 11:48:25 AEST ]]> Preovulatory changes in the levels of three gonadotropin-releasing hormone-encoding messenger ribonucleic acids (mRNAs), gonadotropin beta- subunit mRNAs, plasma gonadotropin, and steroids in the female gilthead seabream, Sparus aurata Tue 16 Oct 2018 11:48:20 AEST ]]> Temporal profile of β follicle-stimulating hormone, β luteinizing hormone, and growth hormone gene expression in the protandrous hermaphrodite, gilthead seabream, Sparus aurata Tue 16 Oct 2018 11:47:44 AEST ]]> Gonadotropin response to GnRH during sexual ontogeny in the common carp, Cyprinus carpio 300%, while that of LHβ increased by 200%. In maturing males, FSHβ mRNA did not change and only a slight increase occurred in that of LHβ. In 16-month-old postvitellogenic females, there was no response of FSHβ mRNA, while that of LHβ dramatically increased. In spermiating males of the same age, mRNA of both FSHβ and LHβ increased following sGnRHa injection. Immunoreactive cGtH was present in the pituitary and plasma of all fish examined, but in juveniles it did not change following sGnRHa injection. In maturing and mature fish of both genders, sGnRHa administration was followed by a marked increase in circulating cGtH, concomitant with a decrease in its pituitary content, indicating the limited amount of the hormone stored in the gland. In conclusion, the response of the gonadotropin subunit mRNAs in the common carp was found to be differential and dependent on the gender and the phase of sexual ontogeny.]]> Tue 16 Oct 2018 11:47:37 AEST ]]> Reproductive development of male and female tilapia hybrids (Oreochromis niloticus × O. aureus) and changes in mRNA levels of gonadotropin (GtH) I and II subunits 10 nM, or E2 at >1 nM) was followed by a decrease in the steady-state levels of the I transcript, while those of II were left unaltered. In situ hybridization studies revealed that in pituitaries of both sexes, the cells producing each of these mRNAs are located in a distinct location. These results suggest that gonadal steroids may exert differential feedback mechanisms at the level of the pituitary to control transcription of each GtH subunit in distinct cell types specific for each hormone.]]> Tue 16 Oct 2018 11:47:33 AEST ]]> Regulation of gonadotropin subunit genes in tilapia Tue 16 Oct 2018 11:44:24 AEST ]]> Detection of Chlamydia trachomatis mRNA using digital PCR as a more accurate marker of viable organism Thu 08 Nov 2018 11:11:25 AEST ]]> Developmental up-regulation and agonist-dependent down-regulation of GABAA receptor subunit mRNAs in chick cortical neurons Thu 07 Sep 2017 10:52:06 AEST ]]>