http://research.usc.edu.au/vital/access/manager/Index ${session.getAttribute("locale")} 5 Molecular analysis of the effect of stressors on oysters http://research.usc.edu.au/vital/access/manager/Repository/usc:18521 Wed 21 Sep 2016 14:09:50 AEST ]]> Molecular signatures of non-typeable Haemophilus influenzae lung adaptation in paediatric chronic lung disease http://research.usc.edu.au/vital/access/manager/Repository/usc:29118 Wed 18 Mar 2020 08:29:57 AEST ]]> Sex-specific transcript expression in the hepatopancreas of the banana shrimp (Fenneropenaeus merguiensis) http://research.usc.edu.au/vital/access/manager/Repository/usc:24605 Wed 18 Mar 2020 08:14:39 AEST ]]> Sex and tissue specific gene expression patterns identified following de novo transcriptomic analysis of the Norway lobster, Nephrops norvegicus http://research.usc.edu.au/vital/access/manager/Repository/usc:23328 600 million reads. De novo assembly that combined the current dataset with two previously published libraries from eyestalk tissue, yielded a reference transcriptome of 333,225 transcripts with an average size of 708 base pairs (bp), with an N50 of 1272 bp. Sex-specific transcripts were detected primarily in gonads followed by hepatopancreas, brain, thoracic ganglia, and eyestalk, respectively. Candidate transcripts that were expressed exclusively either in males or females were highlighted and the 10 most abundant ones were validated via RT-PCR. Among the most highly expressed genes were Serine threonine protein kinase in testis and Vitellogenin in female hepatopancreas. These results align closely with gene annotation results. Moreover, a differential expression heatmap showed that the majority of differentially expressed transcripts were identified in gonad and eyestalk tissues. Results indicate that sex-specific gene expression patterns in Norway lobster are controlled by differences in gene regulation pattern between males and females in somatic tissues. Conclusions: The current study presents the first multi-tissue reference transcriptome for the Norway lobster that can be applied to future biological, wild restocking and fisheries studies. Sex-specific markers were mainly expressed in males implying that males may experience stronger selection than females. It is apparent that differential expression is due to sex-specific gene regulatory pathways that are present in somatic tissues and not from effects of genes located on heterogametic sex chromosomes. The N. norvegicus data provide a foundation for future gene-based reproductive studies. © 2017 The Author(s).]]> Wed 18 Mar 2020 07:48:58 AEST ]]> Transcriptomic changes during previtellogenic and vitellogenic stages of ovarian development in wreckfish (hāpuku), Polyprion oxygeneios (Perciforms) http://research.usc.edu.au/vital/access/manager/Repository/usc:28309 Wed 18 Mar 2020 07:22:36 AEST ]]> Molecular effects of a variable environment on Sydney rock oysters, Saccostrea glomerata: thermal and low salinity stress, and their synergistic effect http://research.usc.edu.au/vital/access/manager/Repository/usc:27779 Wed 18 Mar 2020 07:22:05 AEST ]]> An investigation of sexual reproduction and development in the giant tiger prawn, Penaeus monodon http://research.usc.edu.au/vital/access/manager/Repository/usc:29260 Mon 05 Aug 2019 13:02:43 AEST ]]>