A convenient new analysis of dihydroxyacetone and methylglyoxal applied to Australian Leptospermum honeys

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Title: A convenient new analysis of dihydroxyacetone and methylglyoxal applied to Australian Leptospermum honeys
Author/Creator: Windsor, S
Author/Creator: Pappalardo, M
Author/Creator: Brooks, P R
Author/Creator: Williams, S
Author/Creator: Manley-Harris, M
Abstract: New Zealand mānuka (Leptospermum scoparium) honey is known to exhibit non-peroxide antibacterial activity caused by the active ingredient methylglyoxal which arises by chemical conversion of dihydroxyacetone during honey maturation. This study determines whether methylglyoxal and dihydroxyacetone are present in Australian Leptospermum honeys. This research developed a rapid and sensitive high-performance liquid chromatographic method for the concurrent analysis of methylglyoxal and dihydroxyacetone in honeys. Both compounds were quantified as their O-(2, 3, 4, 5, 6-pentafluorobenzyl) hydroxylamine. HCl derivatives on single run reversed phase high-performance liquid chromatography with diode array detection. Four species of monofloral Leptospermum honeys sourced from Northern Rivers Region, New South Wales, Australia contained methylglyoxal and dihydroxyacetone. The highest methylglyoxal concentrations were found in Leptospermum polygalifolium honeys.
Relation: Journal of Pharmacognosy and Phytotherapy / Vol. 4, No. 1, pp.6-11
Relation: http://dx.doi.org/10.5897/JPP11.025
Year: 2012
Publisher: Academic Journals
Subject: FoR 0607 (Plant Biology)
Subject: FoR 1115 (Pharmacology and Pharmaceutical Sciences)
Subject: FoR 1104 (Complementary and Alternative Medicine)
Subject: high-performance liquid chromatography
Subject: dihydroxyacetone
Subject: methylglyoxal
Subject: Leptospermum
Subject: honey
Subject: manuka

Resource Type: Journal Article
Identifier: URN:ISSN: 2141-2502
Rights: Copyright © 2012 Academic Journals. This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 License.
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