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Inhibition of 5-Lipoxygenase Activity by the Novel Wound-Healing Agent, Opal A
Abstract   Peer reviewed

Inhibition of 5-Lipoxygenase Activity by the Novel Wound-Healing Agent, Opal A

Fraser D Russell, Tanja Windegger, Norman W H Cheetham and G K Mitchell
Wound Repair and Regeneration, Vol.18(4), p.A 68
Australasian Wound and Tissue Repair Society (AWTRS) Meeting, 2nd (Perth, Australia)
2010
DOI: https://doi.org/10.1111/j.1524-475X.2010.00600.x
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https://doi.org/10.1111/j.1524-475X.2010.00600.xView
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Abstract

Clinical Sciences 5-lipoxygenase Opal A wound healing
OPAL A is paw paw pulp treated by a process involving heating and alkalinisation. OPALA can improve resolution of wounds that are resistant to standard care therapy (Mitchell, 2008), although the mechanism is yet to be determined. We hypothesised an anti-inflammatory response to OPALA, and examined this by testing its ability to inhibit 5-lipoxygenase (5-LOX), an enzyme involved in production of pro-inflammatory leukotrienes. 5-LOX converts linoleic acid to a hydroperoxide, an oxidant in the oxidative coupling of 3-methyl-2-benzothiazolinone and 3-(dimethylamino) benzoic acid (Anthon & Barrett, 2001). In the presence of haemoglobin, this reaction produces an indamine dye where development of dye colour (A598) is used as a measure of 5-LOX activity. 5-LOX (630U/ml) was incubated in the absence (control) or presence of OPALA (7.6% solution)1linoleic acid (0.38 mM). 5-LOX activity was observed in controls, and almost abolished by OPALA. To examine the chemical profile of OPALA bioactivity, OPALA (7 ml) was freeze dried, added to silica gel (5 g pre-wet with ethyl acetate) in a column, and eluted sequentially using ethyl acetate, acetone, methanol, and water (40 ml each). Eluants were freeze-dried, reconstituted to 0.5ml in 0.1M phosphate buffer solution, and tested for inhibition of 5-LOX. 5-LOX activity was almost abolished by the methanol and water eluants but unaffected by the other eluants. There was no difference in potency of the methanol and water eluants for inhibition of 5-LOX. In conclusion, OPALA has potential anti-inflammatory activity by inhibiting 5-LOX. Polar compounds in OPALA are mainly responsible for this effect. Mitchell, G. McArthur T, HollerbachK, Richardson M. (2008).AMWA conference abstract, Darwin Anthon & Barrett (2001). J Agric. Food Chem. 49;32-37.

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