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Synphilin-1 and parkin show overlapping expression patterns in human brain and form aggresomes in response to proteasomal inhibition

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- Title
- Synphilin-1 and parkin show overlapping expression patterns in human brain and form aggresomes in response to proteasomal inhibition
- Author/Creator
-
Bandopadhyay, R |
Kingsbury, A E |
Muqit, M M |
Harvey, K |
Reid, A R |
Kilford, L |
Engelender, S |
Schlossmacher, M G |
Wood, N W |
Latchman, D S |
Harvey, Robert J |
Lees, A J
- Description
- Lewy bodies (LBs) are the characteristic inclusions of Parkinson's disease brain but the mechanism responsible for their formation is obscure. Lewy bodies (LBs) are composed of a number of proteins of which alpha-synuclein (α-SYN) is a major constituent. In this study, we have investigated the distribution patterns of synphilin-1 and parkin proteins in control and sporadic PD brain tissue by immunohistochemistry (IH), immunoblotting, and immunoelectron microscopy (IEM). We demonstrate the presence of synphilin-1 and parkin in the central core of a majority of LBs using IH and IEM. Using IH, we show an overlapping distribution profile of the two proteins in central neurons. Additionally, we show sensitivity of both endogenous synphilin-1 and parkin to proteolytic dysfunction and their co-localization in aggresomes formed in response to the proteasome inhibitor MG-132. We confirm that synphilin-1 and parkin are components of majority of LBs in Parkinson's disease and that both proteins are susceptible to proteasomal degradation. © 2005 Elsevier Inc. All rights reserved.
- Relation
- Neurobiology of Disease / Vol. 20, No. 2, pp.401-411
- Relation
- http://dx.doi.org/10.1016/j.nbd.2005.03.021
- Year
- 2005
- Publisher
- Academic Press
- Subject
-
FoR 1103 (Clinical Sciences) |
FoR 1109 (Neurosciences) |
synphilin-1 |
parkin |
lewy bodies |
distribution |
immunohistochemistry |
immunoelectron microscopy |
aggresomes |
proteasome inhibitor |
MG-132 |
yeast two-hybrid analysis
- Resource Type
- Journal Article
- Identifier
- ISSN: 0969-9961
- Reviewed

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