Synphilin-1 and parkin show overlapping expression patterns in human brain and form aggresomes in response to proteasomal inhibition

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Synphilin-1 and parkin show overlapping expression patterns in human brain and form aggresomes in response to proteasomal inhibition

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Title: Synphilin-1 and parkin show overlapping expression patterns in human brain and form aggresomes in response to proteasomal inhibition
Author/Creator: Bandopadhyay, R | Kingsbury, A E | Muqit, M M | Harvey, K | Reid, A R | Kilford, L | Engelender, S | Schlossmacher, M G | Wood, N W | Latchman, D S | Harvey, Robert J | Lees, A J
Description: Lewy bodies (LBs) are the characteristic inclusions of Parkinson's disease brain but the mechanism responsible for their formation is obscure. Lewy bodies (LBs) are composed of a number of proteins of which alpha-synuclein (α-SYN) is a major constituent. In this study, we have investigated the distribution patterns of synphilin-1 and parkin proteins in control and sporadic PD brain tissue by immunohistochemistry (IH), immunoblotting, and immunoelectron microscopy (IEM). We demonstrate the presence of synphilin-1 and parkin in the central core of a majority of LBs using IH and IEM. Using IH, we show an overlapping distribution profile of the two proteins in central neurons. Additionally, we show sensitivity of both endogenous synphilin-1 and parkin to proteolytic dysfunction and their co-localization in aggresomes formed in response to the proteasome inhibitor MG-132. We confirm that synphilin-1 and parkin are components of majority of LBs in Parkinson's disease and that both proteins are susceptible to proteasomal degradation. © 2005 Elsevier Inc. All rights reserved.
Relation: Neurobiology of Disease / Vol. 20, No. 2, pp.401-411
Relation: http://dx.doi.org/10.1016/j.nbd.2005.03.021
Year: 2005
Publisher: Academic Press
Subject: FoR 1103 (Clinical Sciences) | FoR 1109 (Neurosciences) | synphilin-1 | parkin | lewy bodies | distribution | immunohistochemistry | immunoelectron microscopy | aggresomes | proteasome inhibitor | MG-132 | yeast two-hybrid analysis

Resource Type: Journal Article
Identifier: ISSN: 0969-9961
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