Journal article
Cytotoxic-T-lymphocyte-mediated cytolysis of L cells persistently infected with Chlamydia spp
Infection and immunity, Vol.64(6), pp.1944-1949
1996
Abstract
Persistent chlamydial infections have been proposed as a means whereby chlamydiae evade immune resolution of infection. Such a mechanism would require evasion not only of the humoral immune responses but also of cell- mediated immune responses. We hypothesized that if such a mechanism is important, persistently infected cells should not be recognized by cytotoxic T cells. Persistent infections were simulated in vitro by treatment of Chlamydia trachomatis- or Chlamydia psittaci-infected cells with gamma interferon (IFN-?), penicillin, or tryptophan depletion. Cultures were examined for induction of a chlamydial stress response (measured by transcription of groesl RNA) and for the effects on viability, infectivity, morphology, and immune recognition. Although both IFN-? and penicillin induced aberrant chlamydial morphology and growth, we did not find evidence that these treatments elicited a classical stress response. In addition, T- cell-mediated lysis of Chlamydia-infected target cells treated with IFN-? or penicillin or grown in tryptophan-deficient media was examined. The immune cell-mediated lysis of these treated infected cells demonstrated that despite the effects of these compounds on chlamydial growth and development, the infected cells continued to be efficiently recognized and killed by cytotoxic T cells. Thus, it seems unlikely that these in vitro models of persistence represent functional mechanisms to evade immune clearance.
Details
- Title
- Cytotoxic-T-lymphocyte-mediated cytolysis of L cells persistently infected with Chlamydia spp
- Authors
- S J Rasmussen (Author) - Queensland University of TechnologyPeter Timms (Author) - Queensland University of TechnologyP R Beatty (Author) - University of California, United StatesR S Stephens (Author) - University of California, United States
- Publication details
- Infection and immunity, Vol.64(6), pp.1944-1949
- Publisher
- American Society for Microbiology
- Date published
- 1996
- DOI
- 10.1128/IAI.64.6.1944-1949.1996
- ISSN
- 0019-9567; 0019-9567
- Copyright note
- Copyright © 1996 American Society for Microbiology. Reproduced here in accordance with the publishers copyright policy.
- Organisation Unit
- University of the Sunshine Coast, Queensland; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99449212002621
- Output Type
- Journal article
- Research Statement
- false
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