Journal article
Assessment of yellowtail kingfish (Seriola lalandi) as a surrogate host for the production of southern bluefin tuna (Thunnus maccoyii) seed via spermatogonial germ cell transplantation
Reproduction, Fertility and Development, Vol.28(12), pp.2051-2064
2016
Abstract
Germ cell transplantation is an innovative technology for the production of interspecies surrogates, capable of facilitating easier and more economical management of large-bodied broodstock, such as the bluefin tuna. The present study explored the suitability of yellowtail kingfish (Seriola lalandi) as a surrogate host for transplanted southern bluefin tuna (Thunnus maccoyii) spermatogonial cells to produce tuna donor-derived gametes upon sexual maturity. Germ cell populations in testes of donor T. maccoyii males were described using basic histology and the molecular markers vasa and dead-end genes. The peripheral area of the testis was found to contain the highest proportions of dead-end-expressing transplantable Type A spermatogonia. T. maccoyii Type A spermatogonia-enriched preparations were transplanted into the coelomic cavity of 6-10-day-old post-hatch S. lalandi larvae. Fluorescence microscopy and polymerase chain reaction analysis detected the presence of tuna cells in the gonads of the transplanted kingfish fingerlings at 18, 28, 39 and 75 days after transplantation, indicating that the transplanted cells migrated to the genital ridge and had colonised the developing gonad. T. maccoyii germ cell-derived DNA or RNA was not detected at later stages, suggesting that the donor cells were not maintained in the hosts' gonads.
Details
- Title
- Assessment of yellowtail kingfish (Seriola lalandi) as a surrogate host for the production of southern bluefin tuna (Thunnus maccoyii) seed via spermatogonial germ cell transplantation
- Authors
- Ido Bar (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringAndre Smith (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringErin Bubner (Author) - Flinders UniversityGoro Yoshizaki (Author) - Tokyo University of Marine Science and TechnologyYutaka Takeuchi (Author) - Tokyo University of Marine Science and TechnologyRyosuke Yazawa (Author) - Tokyo University of Marine Science and TechnologyBen Nan Chen (Author) - Clean Seas Tuna Ltd, AustraliaScott F Cummins (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringAbigail Elizur (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- Reproduction, Fertility and Development, Vol.28(12), pp.2051-2064
- Publisher
- C S I R O Publishing
- Date published
- 2016
- DOI
- 10.1071/RD15136
- ISSN
- 1031-3613; 1031-3613
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99449596102621
- Output Type
- Journal article
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- Developmental Biology
- Reproductive Biology
- Zoology
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