Interactions of poly(amino acids) in aqueous solution with charged model surfaces - Analysis by colloidal probe

Gregory S Watson; Jolanta A Blach; C Cahill; D V Nicolau; D K Pham; J Wright; S Myhra

doi:10.1016/j.bios.2003.12.017

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Interactions of poly(amino acids) in aqueous solution with charged model surfaces - Analysis by colloidal probe

Journal article

Peer reviewed

Interactions of poly(amino acids) in aqueous solution with charged model surfaces - Analysis by colloidal probe

Gregory S Watson, Jolanta A Blach, C Cahill, D V Nicolau, D K Pham, J Wright and S Myhra

Biosensors and Bioelectronics, Vol.19(11), pp.1355-1362

2004

DOI: https://doi.org/10.1016/j.bios.2003.12.017

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Abstract

double-layer interactions

poly(amino acids)

atomic force microscopy

DLVO model

Biomolecules in a confined solution environment may be subject to electrostatic forces with a range up to 100nm, while van der Waals interaction will account for shorter-range forces. The response of two model poly(amino acids) - poly-L-lysine and poly-L-glutamic acid - has been investigated for a silica/Si-oxide surface at pH 6. The model amino acids were adsorbed, or covalently coupled, to colloidal probes consisting of a microsphere attached to a force-sensing lever. The methodology was based on sensing interaction between the probe and a flat surface through carrying out force versus distance analysis with a scanning force microscope. The results were analyzed within the framework of the conventional DLVO theory. The outcomes illustrate both repulsive and attractive long-range interactions that will hinder, or promote, colloidal biospecies in solution entering the region of attractive short-range interactions at the physical interface. Large 'snap-on' distances were observed for some systems and have been ascribed to compression of the 'soft' functionalized layers. Those observations and measurements of adhesion provided insight into conformation of the adsorbed species and strength of attachment. The results have implications for the efficacy of methods and devices that seek to exploit the properties of micro/nano-fluidic systems. © 2004 Elsevier B.V. All rights reserved.

Details

Title: Interactions of poly(amino acids) in aqueous solution with charged model surfaces - Analysis by colloidal probe
Authors: Gregory S Watson (Author) - Griffith University
Jolanta A Blach (Author) - Griffith University
C Cahill (Author) - Griffith University
D V Nicolau (Author) - Swinburne University
D K Pham (Author) - Swinburne University
J Wright (Author) - Swinburne University
S Myhra (Author) - Griffith University
Publication details: Biosensors and Bioelectronics, Vol.19(11), pp.1355-1362
Publisher: Elsevier BV
Date published: 2004
DOI: 10.1016/j.bios.2003.12.017
ISSN: 0956-5663
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
Language: English
Record Identifier: 99449289802621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Biophysics; Biotechnology & Applied Microbiology; Chemistry, Analytical; Electrochemistry; Nanoscience & Nanotechnology