Resistance-Gene Cassettes Associated With Salmonella enterica Genotypes

Bita Bakhshi; Mohsen Ghafari; Mohammad R Pourshafie; Behnaz Zarbakhsh; Mohammad Katouli; Mohammad Rahbar; Masoud Hajia; Neda Hosseini-Aliabad; Mina Boustanshenas

doi:10.1309/LMFN8D17SOHQHGRP

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Resistance-Gene Cassettes Associated With Salmonella enterica Genotypes

Journal article

Peer reviewed

Resistance-Gene Cassettes Associated With Salmonella enterica Genotypes

Bita Bakhshi, Mohsen Ghafari, Mohammad R Pourshafie, Behnaz Zarbakhsh, Mohammad Katouli, Mohammad Rahbar, Masoud Hajia, Neda Hosseini-Aliabad and Mina Boustanshenas

Laboratory Medicine, Vol.46, pp.90-96

2015

DOI: https://doi.org/10.1309/LMFN8D17SOHQHGRP

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Abstract

salmonella enterica

serogrouping

integrons

genetic diversity

resistance

gene cassettes

Background: The epidemiology of salmonellosis is complex because of the diversity and different serotypes of Salmonella enterica (S. enterica) that occur in different reservoirs and geographic incidences. Objectives: To determine the genotype distribution and resistance-gene content of 2 classes of integron among S. enterica isolates. Methods: Thirty-six S. enterica species were isolated and tested for their serological distribution and the resistance-gene contents of 2 classes of integron, as well as for their genetic diversity, using the pulsed-field gel electrophoresis (PFGE) genotyping method. Results: Serogroups E (36.1%) and D (30.5%) were dominant among the isolates. All of the isolates in serogroup D belonged to the serovar enteritidis. The aadA1 gene was found within all resistance-gene cassettes. We observed 4 common and 26 single pulsotypes among the isolates, which indicated a high degree of genetic diversity among the isolates. Using the PulseNet International standard protocol, it was found that these isolates were different from those reported previously in Iran. Conclusions: The presence of a few common and new pulsotypes among the isolates suggests the emergence and spread of new clones of S. enterica in Iran.

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Title: Resistance-Gene Cassettes Associated With Salmonella enterica Genotypes
Authors: Bita Bakhshi (Author) - Tarbiat Modares University, Iran
Mohsen Ghafari (Author) - Islamic Azad University, Iran
Mohammad R Pourshafie (Author) - Pasteur Institute of Iran, Iran
Behnaz Zarbakhsh (Author) - Pasteur Institute of Iran, Iran
Mohammad Katouli (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Mohammad Rahbar (Author) - Ministry of Health and Medical Education, Iran
Masoud Hajia (Author) - Ministry of Health and Medical Education, Iran
Neda Hosseini-Aliabad (Author) - Islamic Azad University, Iran
Mina Boustanshenas (Author) - Iran University of Medical Sciences, Iran
Publication details: Laboratory Medicine, Vol.46, pp.90-96
Publisher: American Society for Clinical Pathology
Date published: 2015
DOI: 10.1309/LMFN8D17SOHQHGRP
ISSN: 0007-5027
Organisation Unit: University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99449322102621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Medical Laboratory Technology

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