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Cloning and sequencing of dolphinfish (Coryphaena hippurus, Coryphaenidae) growth hormone-encoding cDNA
Journal article   Peer reviewed

Cloning and sequencing of dolphinfish (Coryphaena hippurus, Coryphaenidae) growth hormone-encoding cDNA

A Peduel, Abigail Elizur and Wayne R Knibb
DNA Sequence: journal of DNA mapping, sequencing, and analysis, Vol.5(2), pp.121-123
1994
url
https://doi.org/10.3109/10425179409039713View
Published Version

Abstract

Biochemistry and Cell Biology Genetics Coryphaena hippurus growth hormone sequence fish
The cDNA encoding the preprotein growth hormone from the dolphinfish (Coryphaena hippurus) has been cloned and sequenced. The cDNA was derived by reverse trancription of RNA from the pituitary of a young fish using the method known as Rapid Amplification of cDNA Ends (RACE). An oligonucleotide primer corresponding to the 5' region of Pagrus major and the universal RACE primer enabled amplification using the Polymerase Chain Reaction (PCR). The dolphinfish and yellow-tail, Seriola quineqeradiata, are both members of the sub-order Percoidei (Perciforme) and their GH sequences show a high level of homology.

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Biotechnology & Applied Microbiology
Genetics & Heredity

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