Cytochrome P450 aromatase in grey mullet: cDNA and promoter isolation; brain, pituitary and ovarian expression during puberty

Josephine N Nocillado; Abigail Elizur; A Avitan; F Carrick; B Levavi-Sivan

doi:10.1016/j.mce.2006.08.013

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Cytochrome P450 aromatase in grey mullet: cDNA and promoter isolation; brain, pituitary and ovarian expression during puberty

Journal article

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Cytochrome P450 aromatase in grey mullet: cDNA and promoter isolation; brain, pituitary and ovarian expression during puberty

Josephine N Nocillado, Abigail Elizur, A Avitan, F Carrick and B Levavi-Sivan

Molecular and Cellular Endocrinology, Vol.263(1-2), pp.65-78

2007

DOI: https://doi.org/10.1016/j.mce.2006.08.013

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Abstract

promoter

dopamine

estrogen

real-time quantitative RT-PCR

mullet

In a study towards elucidating the role of aromatases during puberty in female grey mullet, the cDNAs of the brain (muCyp19b) and ovarian (muCyp19a) aromatase were isolated by RT-PCR and their relative expression levels were determined by quantitative real-time RT-PCR. The muCyp19a ORF of 1515 bp encoded 505 predicted amino acid residues, while that of muCyp19b was 1485 bp and encoded 495 predicted amino acid residues. The expression level of muCyp19b significantly increased in the brain as puberty advanced; however, its expression level in the pituitary increased only slightly with pubertal development. In the ovary, the muCyp19a expression level markedly increased as puberty progressed. The promoter regions of the two genes were also isolated and their functionality evaluated in vitro using luciferase as the reporter gene. The muCyp19a promoter sequence (650 bp) contained a consensus TATA box and putative transcription factor binding sites, including two half EREs, an SF-1, an AhR/Arnt, a PR and two GATA-3s. The muCyp19b promoter sequence (2500 bp) showed consensus TATA and CCAAT boxes and putative transcription binding sites, namely: a PR, an ERE, a half ERE, a SP-1, two GATA-binding factor, one half GATA-1, two C/EBPs, a GRE, a NFkappaB, three STATs, a PPAR/RXR, an Ahr/Arnt and a CRE. Basal activity of serially deleted promoter constructs transiently transfected into COS-7, αT3 and TE671 cells demonstrated the enhancing and silencing roles of the putative transcription factor binding sites. Quinpirole, a dopamine agonist, significantly reduced the promoter activity of muCyp19b in TE671. The results suggest tissue-specific regulation of the muCyp19 genes and a putative alternative promoter for muCyp19b.

Details

Title: Cytochrome P450 aromatase in grey mullet: cDNA and promoter isolation; brain, pituitary and ovarian expression during puberty
Authors: Josephine N Nocillado (Author) - Queensland Department of Primary Industries & Fisheries
Abigail Elizur (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
A Avitan (Author) - Hebrew University of Jerusalem
F Carrick (Author) - University of Queensland
B Levavi-Sivan (Author) - Hebrew University of Jerusalem
Publication details: Molecular and Cellular Endocrinology, Vol.263(1-2), pp.65-78
Publisher: Elsevier Ireland Ltd.
Date published: 2007
DOI: 10.1016/j.mce.2006.08.013
ISSN: 0303-7207
Organisation Unit: University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450201602621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Cell Biology; Endocrinology & Metabolism

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