Journal article
Divergent outcomes following transcytosis of IgG targeting intracellular and extracellular chlamydial antigens
Immunology and Cell Biology, Vol.92(5), pp.417-426
2014
Abstract
Antibodies can have a protective but non-essential role in natural chlamydial infections dependent on antigen specificity and antibody isotype. IgG is the dominant antibody in both male and female reproductive tract mucosal secretions, and is bi-directionally trafficked across epithelia by the neonatal Fc receptor (FcRn). Using pH-polarized epididymal epithelia grown on Transwells, IgG specifically targeted at an extracellular chlamydial antigen; the major outer membrane protein (MOMP), enhanced uptake and translocation of infection at pH 6-6.5 but not at neutral pH. This was dependent on FcRn expression. Conversely, FcRn-mediated transport of IgG targeting the intracellular chlamydial inclusion membrane protein A (IncA), induced aberrant inclusion morphology, recruited autophagic proteins independent of lysosomes and significantly reduced infection. Challenge of female mice with MOMP-specific IgG-opsonized Chlamydia muridarum delayed infection clearance but exacerbated oviduct occlusion. In male mice, MOMP-IgG elicited by immunization afforded no protection against testicular chlamydial infection, whereas the transcytosis of IncA-IgG significantly reduced testicular chlamydial burden. Together these data show that the protective and pathological effects of IgG are dependent on FcRn-mediated transport as well as the specificity of IgG for intracellular or extracellular antigens.
Details
- Title
- Divergent outcomes following transcytosis of IgG targeting intracellular and extracellular chlamydial antigens
- Authors
- C W Armitage (Author) - Queensland University of TechnologyC P O'Meara (Author) - Queensland University of TechnologyM C Harvie (Author) - Queensland University of TechnologyPeter Timms (Author) - Queensland University of TechnologyR S Blumberg (Author) - Harvard Medical School, United StatesK W Beagley (Author) - Queensland University of Technology
- Publication details
- Immunology and Cell Biology, Vol.92(5), pp.417-426
- Publisher
- Nature Publishing Group
- Date published
- 2014
- DOI
- 10.1038/icb.2013.110
- ISSN
- 0818-9641; 0818-9641
- Copyright note
- Copyright © 2014 Nature Publishing Group. The accepted version is reproduced here in accordance with the publishers copyright policy. The definitive version is available from http://www.nature.com/icb/
- Organisation Unit
- University of the Sunshine Coast, Queensland; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99448862302621
- Output Type
- Journal article
- Research Statement
- false
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