Immunization with a MOMP-Based Vaccine Pfrotects Mice against a Pulmonary Chlamydia Challenge and Identifies a Disconnection between Infection and Pathology

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Immunization with a MOMP-Based Vaccine Pfrotects Mice against a Pulmonary Chlamydia Challenge and Identifies a Disconnection between Infection and Pathology

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Title: Immunization with a MOMP-Based Vaccine Pfrotects Mice against a Pulmonary Chlamydia Challenge and Identifies a Disconnection between Infection and Pathology
Author/Creator: O'Meara, C P | Armitage, C W | Harvie, M C G | Timms, Peter | Lycke, N Y | Beagley, K W
Description: Chlamydia pneumoniae is responsible for up to 20% of community acquired pneumonia and can exacerbate chronic inflammatory diseases. As the majority of infections are either mild or asymptomatic, a vaccine is recognized to have the greatest potential to reduce infection and disease prevalence. Using the C. muridarum mouse model of infection, we immunized animals via the intranasal (IN), sublingual (SL) or transcutaneous (TC) routes, with recombinant chlamydial major outer membrane protein (MOMP) combined with adjuvants CTA1-DD or a combination of cholera toxin/CpG-oligodeoxynucleotide (CT/CpG). Vaccinated animals were challenged IN with C. muridarum and protection against infection and pathology was assessed. SL and TC immunization with MOMP and CT/CpG was the most protective, significantly reducing chlamydial burden in the lungs and preventing weight loss, which was similar to the protection induced by a previous live infection. Unlike a previous infection however, these vaccinations also provided almost complete protection against fibrotic scarring in the lungs. Protection against infection was associated with antigen-specific production of IFNγ, TNFα and IL-17 by splenocytes, however, protection against both infection and pathology required the induction of a similar pro-inflammatory response in the respiratory tract draining lymph nodes. Interestingly, we also identified two contrasting vaccinations capable of preventing infection or pathology individually. Animals IN immunized with MOMP and either adjuvant were protected from infection, but not the pathology. Conversely, animals TC immunized with MOMP and CTA1-DD were protected from pathology, even though the chlamydial burden in this group was equivalent to the unimmunized controls. This suggests that the development of pathology following an IN infection of vaccinated animals was independent of bacterial load and may have been driven instead by the adaptive immune response generated following immunization. This identifies a disconnection between the control of infection and the development of pathology, which may influence the design of future vaccines. © 2013 O’Meara et al.
Relation: PL o S One / Vol. 8, No. 4, e61962
Relation: http://dx.doi.org/10.1371/journal.pone.0061962
Year: 2013
Publisher: Public Library of Science
Subject: FoR 1108 (Medical Microbiology) | Chlamydia pneumoniae | community acquired pneumonia

Collection(s): Research Publications
Resource Type: Journal Article
Identifier: ISSN: 1932-6203
Rights: Copyright © 2013 O’Meara et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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